Three separate classes of bacterial ice nucleation structures
نویسندگان
چکیده
منابع مشابه
Ice nucleation of bacterial cells
Heterogeneous ice nucleation activity of bacteria: new laboratory experiments at simulated cloud conditions O. Möhler, D. G. Georgakopoulos, C. E. Morris, S. Benz, V. Ebert, S. Hunsmann, H. Saathoff, M. Schnaiter, and R. Wagner Institute for Meteorology and Climate Research (IMK-AAF), Forschungszentrum Karlsruhe, Karlsruhe, Germany Department of Agricultural Biotechnology, Agricultural Universi...
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To mutate the Ice Nucleation Active (INA) gene in Erwinia herbicola strains, Tn-5 transposon carried by Psup2021 plasmid was used. This plasmid was transferred to the bacterial cells by electroporation. Electrotransformation was carried out for 2.5 ms at 1800 v and 1 mm distance between the electrodes. Polymerase chain reaction was used for determination of presence or loss of INA gene, using a...
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The simple linear polymer polyglycerol (PGL) was found to apparently bind and inhibit the ice nucleating activity of proteins from the ice nucleating bacterium Pseudomonas syringae. PGL of molecular mass 750 Da was added to a solution consisting of 1 ppm freeze-dried P. syringae 31A in water. Differential ice nucleator spectra were determined by measuring the distribution of freezing temperatur...
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Bacterial ice nucleation activity (INA+ phenotype) can be traced to the product of a single gene, ina. A remarkably sparse distribution of this phenotype within three bacterial genera indicates that the ina gene may have followed an unusual evolutionary path. Southern blot analyses, coupled with assays for ice-nucleating ability, revealed that within four bacterial species an ina gene is presen...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.5.2521-2526.1990